PTK101
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 | Protein Tyrosine Kinase (EGFR) Assay. The assay was carried out using the Protein Tyrosine Kinase (PTK) Assay Kit (PTK-101). Epidermal growth factor receptor (EGFR; 0.25-8.0 units) was assayed and optical density was measured at 492 nm in a microtiter plate reader. |
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PTK101
Sigma
Protein Tyrosine Kinase (PTK) Assay Kit, Non-Radioactive
sufficient for 96 reactions
Description
| Specificity | Enables the detection, measurement and characterization of protein tyrosine kinase (PTK) activity during purification (column fractions, etc.) of natural and recombinant PTKs, in cell and tissue extracts and immunoprecipitates. This kit is based on an ELISA assay format using the universal PTK substrate poly-Glu-Tyr (PGT). Detection is achieved with a phosphotyrosine specific monoclonal antibody (clone PT-66) conjugated to peroxidase (HRP). EGF Receptor (EGFR) is also included as a positive tyrosine kinase control. |
Properties
| usage | sufficient for 96 reactions |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| Hazard Codes | T,B,N |
| Risk Statements | 20/21-25-36/37/38-40-43-50/53-68 |
| Safety Statements | 26-36/37-45-60-61 |
| RIDADR | UN 3316 6.1/PG 3 |
Components
| Standard component | Adenosine-5'-Triphosphate (ATP 1×5 mg) |
| Epidermal Growth Factor Receptor (EGFR) 1×100 units | |
| Monoclonal Anti-Phosphotyrosine-Peroxidase Conjugate 1 vial | |
| Protein Tyrosine Kinase (PTK) Substrate, polyGlu-Tyr (PGT) 1×5 mg | |
| SIGMA FAST™ OPD 5×5 sets | |
| Tyrosine Kinase Buffer (10x) 1×15 mL | |
| Washing Buffer (PBS-Tween 20) 1 package | |
| 96-well Microtiter Plate, with a strips holder and plate cover 1 each |
